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ORAC,氧自由基抗氧化能力分析试剂盒

发布者:亚博网页版    发布时间:2021-09-09     
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【相关文章】

[1] OxiSelec总抗氧化能力(TAC)分析试剂盒

[2] 超氧化物歧化酶SOD活性分析试剂盒

 

自由基与多种人类疾病的发生发展都有着密切的关系。在清除自由基的过程中,抗氧化剂发挥着重要的作用。因此,了解抗氧化剂的作用及能力就变得至关重要。

 

在评价抗氧化剂能力的诸多方法中,抗氧化能力ORAC(又称,氧自由基吸收能力,Oxygen Radical Absorbance Capacity)分析法,被认定为检测抗氧化剂抗氧化能力的标准方法,是目前普遍被接受的一个标准评价手段。由于该法具有接近机体生理条件、操作简单、认可度和灵敏度高、准确性和重现性佳、高通量、不易受人为因素干扰等优点,被广泛用于农业,食品,保健品,药品及医学领域。

 

自由基.jpg

 

那么如何分析样本中氧自由基抗氧化能力/氧自由基吸收能力(ORAC)?作为专业的生命科学医药原料供应商,亚博网页版为您推荐:众多文章引用的,氧自由基抗氧化能力(ORAC)活性检测试剂盒

产品氧自由基抗氧化能力(ORAC)检测试剂盒
OxiSelect ORAC Activity Assay Kit
货号CBL-STA-345
检测方法荧光法
适用样本类型血清/血浆,尿液,细胞裂解液/提取物,组织裂解物,营养样品(食品,植物等等)
说明书下载点击下载 请于实验前,详细阅读说明书
试剂盒原理ORAC分析法中的自由基主要来源于偶氮化合物2,2'-偶氮-双-(2-眯基丙烷)氯化二氢(2,2'-azobis(2-amidinopropane)dihydrochloride,AAPH)热分解产生的过氧化氢自由基,也可以是芬顿(Fenton)反应过程中产生的羟自由基,以荧光素钠(Sodium Flourescein,FL)为荧光探针,观察自由基与荧光探针作用后,探针荧光强度的衰退过程,以水溶性维生素E类似物(Trolox)作为抗氧化标准物质,检测体系中各种抗氧化剂延缓探针荧光强度衰退的能力,以此评价抗氧化剂的抗氧化能力。
实验优势1 简单快捷:不到2小时完成实验;
2 样本适用:多样性
3 提供标准品;
试剂盒组分1. 96-well Microtiter Plate
2. Fluorescein Probe (100X)
3. Free Radical Initiator
4. Antioxidant Standard (Trolox)
5. Assay Diluent (4X)
保存条件收到后,Fluorescent Probe和Antioxidant Standard (Trolox) 保存于-20℃;其它所有试剂组分保存在4℃。

* 本产品仅适用于科研用途.

 

氧自由基抗氧化能力(ORAC)活性检测试剂盒,原理与标曲展示:

 

氧自由基抗氧化能力(ORAC)活性检测试剂盒-原理示意图.jpg

氧自由基抗氧化能力(ORAC)活性检测试剂盒-原理示意图

 

氧自由基抗氧化能力(ORAC)活性检测试剂盒-标曲示意图.jpg

氧自由基抗氧化能力(ORAC)活性检测试剂盒-标曲示意图

 

FAQ:

 

1.是否可以测试冷冻保存的样品?

RE:通常适用于在 -80℃ 下保存两个月以内样品,但我们建议尽可能测试新鲜样品以获得最佳结果

 

2.ORAC、HORAC和TAC检测之间的区别,如何选择?

RE:所有三种测定都测量抗氧化能力,但略有不同。ORAC检测分析氧自由基的抗氧化能力;HORAC检测分析羟基自由基的抗氧化能力;TAC基于单电子转移(SET)机制和铜离子,检测总体抗氧化能力。总体而言,研究人员更偏好选择ORAC分析而不是HORAC,因为过氧自由基似乎比羟基自由基更相关。

 

3.更多常见问题,请点击:cellbiolabs.com/faq/oxidative-stress-faq/orac-assay

 

近期发表文章(仅摘取部分展示):

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Yuasa, M. et al. (2020). Antioxidant and taste properties of fresh onion (Allium cepa L.) leaves. J. Food Meas. Charact. doi: 10.1007/s11694-020-00704-w.

Wake, H. et al. (2020). Histidine-rich glycoprotein possesses anti-oxidant activity through self-oxidation and inhibition of hydroxyl radical production via chelating divalent metal ions in Fenton's reaction. Free Radic Res. doi: 10.1080/10715762.2020.1825703.

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